This protocol is designed for purification of total dna from grampositive bacteria. No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic, prognostic, therapeutic, or blood banking. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of. Efficient lysis with no mechanical homogenization only 20 minutes of total handson preparation time purification of highquality dna from a wide range of samples the qiaamp dna mini kit can be used to isolate dna from human tissues, swabs, blood or washed cells from urine. For purification of up to 600 micro g genomic, mitochondrial, or viral dna from blood. For purification of up to 12 micro g genomic, mitochondrial, or viral dna from blood and related body fluids. Qiaamp dna blood mini kit 250, 51106 from qiagen labsave. Incubate at room temperature 1525c for 1 minute, and then centrifuge at 6000 x g 8000 rpm for 1 minute. Compatibility of the qiagen qiaamp dna blood mini kit with.
Also covered, was the possibility of using cfdna to assist with breast cancer screening, in individuals who had been recalled for suspicious mammograms. Quantitation of viral rna and proviral dna viral rna load in plasma was determined at a re. Euresist qiaamp viral rna mini kit qiagen rna extraction. However, due to the large solution volumes used, the qiavac connecting system is required. Timerun, 4 to 12g yield, for purification of up to 12g. Laboratory protocol for dna extraction from oracollectdx. Dna isolation from whole blood pdf translational research. In this study, we used human blood, buffy coat and a total of 76 simulated touch dna samples to test the effectiveness of the following five common dna extraction methods, namely, chelex 100, qiaamp dna blood mini kit, qiaamp dna investigator kit, qiasymphony dna investigator kit and dna iq system, in the recovery of such dna. Pathogen dna purification from swabs or 400 l blood cotton swabs or whole blood samples were spiked with a mixture of. The chemagic viral dnarna kit was evaluated for extraction of cytomegalovirus cmv, hepatitis b virus hbv, and hepatitis g virus hgv by using the qiaamp dna blood mini kit and the. Comparative study of dna isolated from whole blood using. Qiaamp dna mini and blood mini handbook 112007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use.
Lyophilized qiagen protease can be stored at room temperature 1525c for up to 6 months without any decrease in performance. Isolation of total rna from iswabrna using the qiaamp. Qiagen qiaamp rna blood mini kit, 50 preps, 50 to 1. Qiaamp dna blood maxi kit 50, 51194 from qiagen labsave. Briefly, for cat, dog, and horse samples, 200 mg of feces was. Possible involvement of distinct phylogenetic clusters of. Qiaamp dna stool mini kit handbook 082001 qiaampdna stool procedure qiaamp dna stool mini kit handbook 082001 10 purification qiaampspin columns qiaampdna purification. Qiagen qiaamp dna ffpe tissue handbook en qiaamp dsp dna ffpe tissue kit handbook. Dna is extracted from peripheral blood using qiaamp dna mini kit qiagen in accordance to the blood and body fluid protocol of the user manual supplied with. These products are not intended for the diagnosis, prevention, or treatment of a disease. Set the water bath to 70oc and thaw cell pellets on ice. For purification of up to 12 g genomic, mitochondrial, or viral dna from blood and related body fluids.
Dna extraction protocol for iswabdna using qiaamp dna mini. Standard operating procedure sop for dna extraction with qiaamp mini kit i. This protocol is a summary of the one given with the kit. Extraction of dna from blood samples using qiaamp dna mini. If you have any questions or experience any difficulties regarding qiaamp dna mini or. Mar 27, 2016 video tutorials of the split procedure. The purified dna is up to 50 kb in size, with fragments of 20 30 kb predominating. Refer to the handbook for protocols for swabs, dried blood spots, cultured cells, fixed tissue, bacteria, yeast or other material. What is the best kit or protocol for dna genome extraction. Dna isolation will be performed using the qiaamp dna mini kit from qiagen cat no. Jul 02, 2016 related with qiaamp dna mini kit and qiaamp dna blood mini kit. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from. Carefully open the qiaamp mini spin column and add 200 l buffer ae or distilled water.
Qiaamp circulating nucleic acid kit, demonstrating that the kits give comparable sequence profiles. For purification of cellular rna from fresh whole blood. View qiaamp dna mini kit gdna extraction kit manual. The results showed that blood dna extracted using qiaamp dna blood mini kit was found to be the most effective and consistently produced high concentrated and pure dna for three animal samples. Follow instructions of the protocol dna purification from blood or body fluids spin protocol or the protocol dna purification from blood or body fluids vacuum protocol in the qiaamp dna mini and. For dna purification from whole blood, plasma, serum, buffy coat, lymphocytes, dried blood spots qiaamp. Dna yields from white blood cells are expected to be up to 50. Qiaamp dna blood mini kit 50 from qiagen selectscience. Standard operating procedure sop for dna extraction with.
The purpose of this study is to compare four commercially available blood dna isolation methods. The qiaamp dna mini kit provides a fast and easy method for the purification of. Qiagen qiaamp dna micro kit, 50 preps, 1 to 100l sample, 20 to 100l elution volume, whole blood sample, silica technology, manual processing, spin column format, genomic dna, mitochondrial dna purification, 30 min. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed ce.
Extraction of dna from blood samples using qiaamp dna mini kit. Dna is extracted from peripheral blood using qiaamp dna mini kit qiagen in accordance to the blood and body fluid protocol of the user manual supplied with the kit. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. The qiaamp cador pathogen mini kit is intended for the extraction of pathogen nucleic acids viral rna and dna and bacterial dna from animal whole blood, serum, plasma, other body fluids, swabs. Qiaamp dna mini and blood mini handbook 062012, protocol. Pdf comparison of dna concentration and purity of animal.
The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use. Qiagen qiaamp dna micro kit, 50 preps, 1 to 100l sample, 20 to 100l elution. Extraction of dna from formalinfixed, paraffinembedded ffpe tissue is a critical kit zymo research corp and the qiaamp dna ffpe tissue kit qiagen. To set up and operate the qiavac 24 plus for a particular application, refer to. No claim or representation is intended for their use to. Standard operating procedures clinical protocol v dna isolation. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Effective mechanical sample lysis for reliable pathogen. Supp prot saliva dna qiagen qiaamp blood mini kit v1. Qiagen qiaamp dna blood mini kit, 50 preps, 1 to 200l. Qiagen qiaamp dna ffpe tissue handbook teukumrelac.
Microbial dna was isolated using the qiaamp ucp pathogen mini kit, the qiaamp ucp purepathogen blood kit or the qiaamp dna mini kit, according to the protocols described in the respective kit handbooks. Scope and purpose qiagen qiaamp kits provide a fast, easy, and reliable method to purify total dna from small quantities of human tissue or blood. No mechanical homogenization is necessary as the tissues are lysed enzymatically. Qiagen qiaamp dna blood mini kit, 50 preps, 1 to 200l sample, 50 to 200l elution volume, whole blood, body fluids sample, spin column format, silica technology, manual processing, 20 to 40 min. We recommend all users of qiagen products to adhere to the nih guidelines. Qiaamp dna mini kit and qiaamp dna blood mini kit 092001 7 product use limitations qiaamp kits are intended as generalpurpose devices. Qiaamp dna mini kit nucleic acid purification bacterial. Qiagen qiaamp dna blood maxi kit, 50 preps, 3 to 10ml sample, 500 to. All due care and attention should be exercised in the handling of the products. Third edition june 2012 qiaamp dna mini and blood mini handbook for dna purification from. Lyophilized qiagen protease can be stored at room temperature 1525c for up to 12 months without any decrease in performance. Efficient lysis with no mechanical homogenization only 20 minutes of total handson preparation time purification of highquality dna from a wide range of samples the. An evaluation of the performance of five extraction methods.
Qiaamp dsp dna blood mini kit handbuch 50 pdf free download. Qiaamp dna mini kit bacterial or genomic dna purification from water, filters, or surface swabs by qiagen. Qiaamp dna mini and blood mini handbook moodle ufsc. The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for molecular biology applications. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed cells from urine. Dna extraction was performed following the manufacturers recommendations. Third edition june 2012 qiaamp dna mini and blood mini handbook for dna purification. Qiaamp viral rna mini kit qiagen rna extraction protocol. Related with qiaamp dna mini kit and qiaamp dna blood mini kit.
Qiaamp rna blood mini kit 50 52304 from qiagen sample to. Qiaamp dna stool mini kit 50 from qiagen selectscience. Qiaamp midi and maxi spin columns can also be processed using the qiavac 24 plus. Efficient extraction of viral dna and viral rna by the. The rneasy mini kit, rneasy protect mini kit including rna later rna stabilization reagent, and rneasy plant mini kit should be stored dry at room temperature 1525c and are stable for at least 9 months under these conditions. Qiaamp dna micro kit 50 56304 from qiagen sample to. White blood cell pellet suspended in hanks balanced salt solution hbss. The qiaamp dna stool mini kit provides silica membranebased purification of up to 30. Please be sure to read the qiaamp rna blood mini kit handbook before. The convenient spincolumn procedure reduces handson preparation time to 20 minutes. Follow instructions of the protocol dna purification from blood or body fluids spin protocol or the protocol dna purification from blood or body fluids vacuum protocol in the qiaamp dna mini and blood mini handbook, starting with step 3, where 200. Further information qiaamp dna mini and blood mini handbook. For purification of genomic and mitochondrial dna from small samples.
706 141 250 155 953 380 371 552 981 190 315 1271 472 542 317 108 161 1005 871 732 1356 741 1005 385 1202 910 987 711 718 1343 1021 34 794 215 48 1209 839 333 240